The changes in histopathology of various organs and growth inhibition´:of the chick embryos incubated with radioactive sulfur (35S) were experimentally studied.
The various doses of ´5S were injected_ into the yolk sac at different intervals and `the weight changes of the embryos were evaluated to determine the growth inhibition´ rates. 41
The embryos sacrified on various, incubation days were used for ´the study of histopathological ´ changes in organs such as the bone,´ liver, kidney, gonad, and eye.´
Following were the results:´
1) The weight changes of the´ 35S treated groups were as follows: i. Embryos treated on the 5 th incubation day:
No weight .changes were _noted on the 8 th incubation day, ` ´however, the´ growth``´ h
th inhibition:
rate of 32.1% was noted in the group treated with 50,uC and of 38.2 % in the ´group treated.
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with 150 pC on the 12th incubation day. The rates were 9..1 and 12.1 % on the 15th incubation day, and 6.5 and 10.6% on the 18th incubation day respectively. ´ ii. Embryos treated on the 8th incubation day:
The growth inhibition rates on the 12th, 15th and 18th incubation days in the groups treated with 50 pC were 20.9, 25.9 and 18.8% and in those treated with 150 UC were 20.0, 14.9 and 16.9% respectively.
iii. Embryos treated on the 12th incubation day:
The growth inhibition rates on the 15th and 18th in the groups treated with 50pC were 13.6 and
21.1 % and in those treated with 150 PC were 26.7 and 6. 5% and in those treated with 250 pC were 10.6 and 12.6% respectively.
iv. Embryos treated on the 15th incubation day:
The growth inhibition rates on the 18th in the groups treated with 50 μC were 6.5 % and in those treated with 150 pC were 10. 1% and in those treated with 250 pC were 8.5% respectively.
In summary, the longer the incubation days, the less the growth inhibition rates.
II) The histopathological changes in the vprious organs were as follows:
i. Bone: Hyperplasia and edematous changes of the bone cavity, irregular distribution of immature granular cells and increased number of the myeloblast, megakaryocyte and reticuloendothelial cells were noted.
ii. Liver: The embryos treated with 150 μC of 35S on the 8th incubation day showed necrosis and nucleolysis of the liver cell and abnormal enlargement of sinusoid on the 12th incubation day. The longer the incubation days, the more severe the changes such as the pyknotic artophy of the liver cells and heterochromatism.
The embryos treated on the 5th incubation day with 50 and 150 μC of 35S showed little changes, but sight enlargement and accumulation of serous fluid in the sinusoid, on the 8th incubation day.
iii. Kidney: No particular changes except atrophic changes of epithelium were noted in early stage, however, the infiltration of the granular cell and monocyte into the cortex and pyknotic changes of vascular glomeruli were noted in later stage.
These changes were not closely related to the doses of 3SS given.
iv. Gonad: The degenerative changes such as destruction of the immature germ cells, hyperplasia and vacuolization of the stroma were noted in testis and ovary.
v. Eye: A slight distortion of the cornea and sclera was noted. The hypertrophy of inner layer and blood cell infiltration into the vascular layer of the choroid membrane were noted in embryo
groups on . the 12, 15 and 18th incubation days.
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